Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Optimal dilution of the MSH2 antibody should be determined by the researcher.

Recombinant full-length human MSH2 protein was used as the immunogen for the MSH2 antibody.

Aliquot the MSH2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.